Intermediate 15 min read

How to Build a Longevity Supplement Stack

Learn how to build an effective longevity supplement stack. Covers NMN, resveratrol, quercetin, and other key compounds for anti-aging and healthspan.

Dr. Sarah Mitchell , Longevity Researcher, PhD
Published: January 22, 2025
Updated: January 22, 2025

If you’re serious about extending your healthspan and potentially your lifespan, you’ve probably wondered how to combine different longevity supplements for maximum effect. The truth is, individual supplements can be powerful, but combining them strategically creates synergistic effects that may amplify their benefits.

This guide walks you through building a longevity supplement stack from the ground up.

Important note: This guide is for educational purposes only. Always consult with a healthcare provider before starting any supplement regimen.

Why Stack Supplements? Understanding Synergy

Synergy occurs when two or more compounds produce effects greater than the sum of their individual effects. In longevity science, this happens because aging involves multiple interconnected pathways:

  • NAD+ decline - Affects cellular energy and repair
  • Cellular senescence - Accumulation of damaged “zombie” cells
  • Mitochondrial dysfunction - Reduced energy production
  • Chronic inflammation - Systemic damage over time
  • Epigenetic drift - Changes in gene expression patterns

A well-designed stack addresses multiple mechanisms simultaneously.

Real-World Example: NMN and Resveratrol

Dr. David Sinclair has publicly discussed taking NMN with resveratrol:

  • NMN boosts NAD+ levels, providing fuel for sirtuins
  • Resveratrol activates sirtuins, particularly SIRT1

Combining them gives you both fuel and activation, potentially amplifying longevity benefits.

The Core Longevity Stack: NMN + Resveratrol + TMG

NMN (Nicotinamide Mononucleotide)

What it does: Direct precursor to NAD+, essential for cellular energy production, DNA repair, and sirtuin function.

Typical dosage: 250-1000mg daily

Best time to take: Morning

Key considerations:

  • Look for third-party tested products
  • Sublingual forms may offer better absorption
  • Store in a cool, dry place

Resveratrol

What it does: Activates SIRT1 and other sirtuins, has antioxidant and anti-inflammatory properties.

Typical dosage: 250-1000mg daily

Best time to take: Morning with a fat source (resveratrol is fat-soluble)

Key considerations:

  • Trans-resveratrol is the active form
  • Take with olive oil, yogurt, or fatty breakfast

TMG (Trimethylglycine/Betaine)

What it does: Methyl donor that replenishes methyl groups consumed when NMN converts to NAD+.

Typical dosage: 500-1000mg daily

Best time to take: With your NMN

Key considerations:

  • Particularly important at higher NMN doses (500mg+)
  • Supports healthy homocysteine levels

Adding Quercetin: Senolytic Power

Understanding Senolytics

Senescent cells are damaged cells that accumulate in tissues and secrete inflammatory compounds. Senolytics selectively eliminate these cells.

Why Quercetin?

Quercetin is a flavonoid with demonstrated senolytic activity, especially in combination with other compounds like fisetin.

Typical dosage: 500-1000mg daily, or higher doses intermittently

Best time to take: With meals

Quercetin Protocols

Daily maintenance: 250-500mg daily

Periodic senolytic protocol: 1000-1500mg daily for 2-3 consecutive days, repeated monthly

Vitamin D3 + K2: The Essential Duo

Vitamin D3

Vitamin D deficiency affects an estimated 40% of Americans. Beyond bone health, it influences immune function, mood, and gene expression.

Typical dosage: 2000-5000 IU daily

Best time to take: Morning with food containing fat

Why K2 Matters

K2 ensures calcium goes to bones rather than accumulating in arteries.

Typical dosage: 100-200mcg of MK-7 form daily

Best time to take: With your D3

Omega-3 Fatty Acids

How Omega-3s Support Longevity

  • Inflammation reduction: Help resolve chronic inflammation
  • Brain health: DHA is a major component of brain tissue
  • Cardiovascular protection: Support healthy lipid levels
  • Telomere maintenance: May help preserve telomere length

Typical dosage: 2-3 grams combined EPA/DHA daily

Best time to take: With meals

Quality Matters

Look for:

  • Third-party testing (IFOS certification)
  • Triglyceride form (better absorbed)
  • Low oxidation levels
  • Sustainable sourcing

Optional Advanced Additions

Fisetin

Like quercetin, fisetin is a senolytic flavonoid. Research suggests it may be even more potent at clearing senescent cells.

Typical dosage: 100-500mg daily or higher doses periodically

Spermidine

Induces autophagy, your body’s cellular recycling process.

Typical dosage: 1-5mg daily

Berberine

Activates AMPK, supports healthy blood sugar and lipid levels.

Typical dosage: 500mg two to three times daily

Note: May interact with medications

CoQ10 (Ubiquinol)

Supports mitochondrial function and energy production.

Typical dosage: 100-200mg of ubiquinol form daily

Timing and Dosing Schedule

Morning Stack (with breakfast)

SupplementDoseNotes
NMN250-500mgNAD+ is higher in morning
Resveratrol250-500mgNeeds fat for absorption
TMG500-1000mgSupports NMN metabolism
Vitamin D32000-5000 IUFat-soluble
Vitamin K2100-200mcgTake with D3
Omega-31-2g EPA/DHAWith food

With Lunch or Dinner

SupplementDoseNotes
Quercetin500mgBetter absorbed with food
Additional Omega-31g EPA/DHAIf splitting dose

Budget Considerations

Starter Stack (Under $75/month)

SupplementMonthly Cost
Vitamin D3 + K2$10-15
Omega-3$20-30
NMN (250mg/day)$25-40

Intermediate Stack ($100-150/month)

Add:

  • Resveratrol (500mg): $20-30
  • TMG: $10-15
  • Quercetin: $15-20

Advanced Stack ($200-300/month)

Add:

  • Higher-dose NMN (1g): Additional $30-50
  • Fisetin: $25-35
  • Spermidine: $30-50
  • CoQ10: $25-40

Money-Saving Tips

  1. Buy in bulk for discounts
  2. Powder forms are often cheaper
  3. Prioritize quality over quantity
  4. Watch for sales

What to Avoid

Redundant Combinations

  • NMN and NR together: Both are NAD+ precursors; choose one
  • Multiple forms of the same antioxidant: Usually unnecessary
  • Excessive vitamin E: High-dose supplementation has shown concerning results

Potential Interactions

  • Resveratrol and blood thinners: Use caution
  • Berberine and medications: Can interact with many drugs
  • Quercetin and thyroid medications: Separate by several hours

Sample Stacks by Goal

The “Sinclair-Inspired” Stack

  • NMN: 1g daily
  • Resveratrol: 1g daily with yogurt
  • TMG: 500mg-1g daily
  • Vitamin D3: 4000-5000 IU daily
  • Vitamin K2: 180-200mcg daily
  • Omega-3: 2-3g EPA/DHA daily

The “Cellular Cleanup” Stack

  • Quercetin: 500mg daily + 1500mg for 2 days monthly
  • Fisetin: 100mg daily + 1-2g for 2 days monthly
  • Omega-3: 2g EPA/DHA daily
  • Vitamin D3 + K2: Standard doses

The “Metabolic Health” Stack

  • NMN: 500mg daily
  • Berberine: 500mg twice daily
  • CoQ10 (ubiquinol): 200mg daily
  • Omega-3: 2-3g EPA/DHA daily
  • Vitamin D3 + K2: Standard doses

Safety Considerations

Before Starting

  1. Get baseline bloodwork
  2. Consult your healthcare provider
  3. Start slowly - one supplement at a time
  4. Track how you feel

Monitoring Progress

Consider retesting every 6-12 months:

  • Vitamin D levels
  • Lipid panel
  • Fasting glucose and HbA1c
  • Inflammatory markers (hs-CRP)

The Bigger Picture

Supplements work best alongside:

  • Regular exercise: Both resistance and cardio
  • Quality sleep: 7-9 hours
  • Healthy diet: Whole foods, adequate protein
  • Stress management: Chronic stress accelerates aging
  • Social connection: Important for longevity

No supplement stack can compensate for poor lifestyle choices.

Building Your Personal Protocol

Start with the basics: vitamin D, omega-3s, and one NAD+ precursor. Once established, gradually expand based on your goals, budget, and response.

The goal isn’t to take the most supplements - it’s to take the right ones, consistently over time.


This guide is for educational purposes only and does not constitute medical advice. Always consult with a qualified healthcare provider before starting any supplement regimen.

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